Figure 1. In vitro acetylation and methylation assays using synthetic Tat peptides.
(A) In vitro methylation assays of Tat ARM peptides (aa 45–58). Unmodified, K50-acetylated or K51-methylated peptides were incubated with recombinant SET7/9/KMT7 and 3H-radiolabeled S-adenosyl-L-methionine (SAM). Peptides were separated by Tris-Tricine gel electrophoresis and visualized by autoradiography. A quantification of band intensities of three independent experiments is shown below. (B) In vitro acetylation assays of Tat ARM peptides. Unmodified, K50-acetylated or K51-methylated peptides were incubated with recombinant p300-HAT and 14C-acetyl coenzyme A. Peptides were processed as in A. A quantification of band intensities of three independent experiments is shown below.