Table 1.
MS/MS Experimental Conditions for Specific Reaction Monitoring (SRM) and Information-Dependent Acquisition-Enhanced Product Ion (IDA-EPI)
| Analyte | Q1 (m/z) | Q3 (m/z) | CEa (V) | EPb (V) | DPc (V) | CXPd (V) | |
|---|---|---|---|---|---|---|---|
| 1, 3, and 5 | 358 | 155 | 37 | 10 | 86 | 12 | |
| 2 | 372 | 155 | 37 | 10 | 86 | 12 | |
| 4 and 6 | 344 | 155 | 35 | 10 | 71 | 12 | |
| SRM | 2-d4 (IS) | 376 | 155 | 37 | 10 | 86 | 12 |
| 5-d5 (IS) | 363 | 155 | 37 | 10 | 86 | 12 | |
| 1-,3-, and 5-Gluc | 534 | 358 | 30 | 10 | 30 | 15 | |
| 2-Gluc | 548 | 372 | 30 | 10 | 30 | 15 | |
| 4- and 6- Gluc | 520 | 344 | 30 | 10 | 30 | 15 | |
|
| |||||||
| IDA-EPI | 1–6 | [MH]+ | 80–600 | 40 | 10 | 40 | NA |
a
CE, Collision Energy;
b
EP, Entrance Potential;
c
DP, Declustering Potential;
d
CXP, Collision Cell Exit Potential