FIGURE 8.
Comparison of data for striatum and cortex/hippocampus. (A) Pairwise scatter plot comparing miRNA synaptic enrichment ratios from Lugli et al. 2008 (adult mouse cortex and hippocampus; measured by microarray) with RNA-Seq data (adult mouse striatum) from these experiments (Lugli et al. 2008). Horizontal axis, data from Lugli et al. 2008 computed as ratio of PSD (Syn)/total tissue (Tot) expression; vertical axis, data from RNA-Seq computed as PSD/NAc lysate ratio of average of saline and cocaine expression levels for adult mouse striatal PSD and NAc lysate. Blue circles were identified as cocaine-regulated and/or were assayed by qPCR, green circles indicate miRNAs enriched at striatal PSDs by RNA-Seq, red circles indicate miRNAs depleted at striatal PSDs by RNA-Seq, and gray circles represent all other miRNAs. Size of circle is proportional to average RNA-Seq expression value. (B) Synaptosomal enrichment and depletion categories for miRNAs from Siegel et al. 2009 as determined by microarray analysis of rat forebrain samples (Siegel et al. 2009). Heat map shows high-throughput sequencing data for saline- and cocaine-treated NAc lysates and striatal PSDs of wild-type mice; normalized miRNA frequency was calculated and Z-score was computed on normalized miRNA frequency across all samples. Blue indicates low expression and yellow indicates high expression.