Figure 2.
Depletion of Cog6 impairs the Golgi localization of TGN46, CI-MPR, and γ-adaptin. (A and B) HeLa cells were transiently transfected with a Cog6 shRNA construct (A) or cotransfected with the Cog6 shRNA construct together with a vector encoding EGFP (B). 72 h later, the cells were fixed and double immunostained with an antibody against TGN46, CI-MPR, or γ-adaptin and either the anti-Cog6 antibody (A) or anti–Golgin 97 antibody (B). The TGN localization of TGN46, CI-MPR, or γ-adaptin was determined by colocalization with Golgin 97 (B). Cog6-depleted cells, which are marked by arrowheads, were identified either by immunostaining with the anti-Cog6 antibody (A) or by EGFP expression (B). Bars, 10 µm.