FIGURE 2.
Identification of the sequence elements within Nrl promoter/enhancer required for rod-specific expression in vivo. Diagram of the upstream region of Nrl displays three main conserved clusters C, B, and A. Representative sections from mouse retina, transfected in vivo with the ubiquitin-GFP construct (A–H) and Nrl promoter-mCherry (A′–H′), are shown. Retinas were harvested at P14 for examining the expression of the reporter gene. (A″–H″) are merged images. Green, GFP fluorescence; red, mCherry fluorescence; blue, DAPI staining. ub-GFP, ubiquitin-GFP; Nrlp-mCh, Nrl promoter-mCherry. Scale bar, 20 μm.