Figure 8.
PROTOMAP and Subtiligase provide complementary and synergistic information content-mapping low-magnitude cleavage events in abundant proteins. In this example, it is clear from the peptograph of MYH9 in the soluble fraction that the magnitude of cleavage is small and obscured by the bleeding of this abundant protein into lower molecular weight regions of the gel. An N-terminope was nonetheless detected in the middle of the protein by the Subtiligase method, thereby confirming proteolysis (and persistent fragments corresponding to this site of cleavage can be observed in the particulate fraction; right peptograph). The peptographs generated by PROTOMAP confirm this protelytic event as minor in magnitude with the majority of the parent protein remaining intact.