Figure 3.
Analysis of Lin−Sca1+CD49f+cell (LSC) grafts for β-catenin and prostate epithelial markers. (A): β-Catenin immunostaining. Arrow, nuclear β-catenin. Scale bar = 50 µm. (B): p63 immunostaining. Arrows, p63+ cells in control acini. Graph: average of four fields. Scale bar =100 µm. (C): LSC grafts stained with cytokeratin 5 (CK5) and CK8 antibodies. Arrows, CK5/CK8 double positive cells. Scale bar = 50 µm. Abbreviations: CK, cytokeratin; DAPI, 4′,6-diamidino-2-phenylindole; LV, lentivirus; β-Cat*-LV, LV-mediated transduction of constitutively active β-catenin.