Figure 3. Hepatocyte-specific Mdm2 deletion induces modest hepatocyte apoptosis.
(A–E) Mdm2fl/flalb-cre [Cre(+)] mice and Mdm2fl/fl [Cre(–)] mice were examined at 6–8 weeks of age; more than 6 mice per group. (A) Hepatocyte apoptosis was evaluated by H&E staining and TUNEL staining of liver sections; black arrows indicate TUNEL-positive cells. Original magnification, upper panels, ×400; lower panels, ×200. (B) TUNEL-positive cell counts of liver sections. (C) Serum levels of ALT. (D) Expression of cleaved caspase-3 protein in the liver sections was assessed by immunohistochemistry; black arrows indicate cleaved caspase-3–positive cells. Original magnification, ×400. (E) Serum caspase-3/7 activity. RLU, relative light units.