Fig. 4.
Colocalization of α-Synuclein with the phosphatidic acid marker Spo2051–91 in S. cerevisiae. (a) Fluorescence microscopy of cells expressing α-synuclein-EGFP and RFP-Spo2051–91. α-Synuclein-EGFP colocalizes with RFP-Spo2051–91 at the plasma membrane and in early accumulations. As the accumulations mature to large cytoplasmic accumulations (lower panels), they no longer colocalize with RFP-Spo2051–91. (b) Fluorescence microscopy of cells expressing EGFP, 58–140-EGFP, β-synuclein-EGFP, or Δ71–82-α-syn-EGFP and RFP-Spo2051–91. EGFP and 58–140-EGFP do localize to the cytoplasm and do not colocalize with RFP-Spo2051–91. β-Synuclein-EGFP and Δ71–82-α-syn-EGFP colocalize with RFP-Spo2051–91 at the plasma membrane, but do not form large cytoplasmic accumulations. Scale Bar, (a) 1 μM