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. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: Cell. 2011 Jul 8;146(1):92–104. doi: 10.1016/j.cell.2011.06.005

Figure 3. The Med26 NTD is needed for Mediator-dependent recruitment of ELL/EAF but not TFIID to promoters.

Figure 3

(A) Schematic of immobilized template assay. (B) Med26 NTD is dispensable for Mediator-dependent recruitment of TFIID to a promoter. Assays contained GAL4×5-MLT template and GAL4-VP16, TFIID, and Mediator (Med) with Med26 or Med26ΔNTD as indicated. (C) Mediator-dependent recruitment of ELL/EAF1 to GAL4- (lanes 1-3) or HNF4α-responsive promoters. Med(F-Med26 wt), Med26-Mediator. (D) Med26ΔNTD-Mediator [Med(F-Med26 ΔNTD)] does not recruit EAF1 to a promoter. (E) Free Med26 is not sufficient for EAF1 recruitment. Lanes 1-4, assays contained either free full length Med26 or Med26-Mediator. Lanes 5-8 show amounts of free Med26 and Med26-Mediator in binding assays. (F) Free Med26 NTD blocks Mediator-dependent EAF1 recruitment. Assays were performed with Med26-Mediator, with or without free Med26 NTD (His-Med26-NTD).