FIGURE 5.
IFT52(281–381) binds to IFT88. a, GST pulldowns showing a direct interaction between GST-IFT52(281–381) and full-length (f.l.) SUMO-IFT88 after co-expression in E. coli. The first four lanes show total bacterial lysate, and the last three lanes show the GST pulldowns between GST-IFT52(1–281), GST-IFT52(281–381), and GST-IFT52(382–454) with SUMO-IFT88. Degradation fragments are marked with asterisks. b, Superdex200 chromatogram and Coomassie Blue-stained SDS-polyacrylamide gel of the peak fractions showing the purification of a SUMO-88/GST-IFT52(281–381) complex. Elution volumes analyzed by SDS-PAGE are indicated with a bar below the chromatogram. Degradation fragments of SUMO-88 are marked with asterisks. In both a and b IFT88 was expressed from the pEC-A-SUMO vector, and the IFT52 fragment was expressed from the pEC-K-GST vector.