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. 2011 Jun 3;286(30):26344–26352. doi: 10.1074/jbc.M111.254920

FIGURE 1.

FIGURE 1.

Co-expression and purification of recombinant IFT88/70/52/46, IFT70/52/46, and IFT70/52(1–381) complexes. a, overview of the purification procedure including Ni2+-NTA, glutathione-Sepharose, heparin, ion-exchange (Mono Q), and size-exclusion chromatography. The vectors used for co-expression of these proteins were pEC-K-GST-IFT88, pEC-S-His-IFT70, pEC-A-His-IFT52, and pETMCN-IFT46. TEV, tobacco etch virus. b, HiLoad Superdex200 chromatogram and Coomassie Blue-stained SDS-polyacrylamide gel of the peak fractions, showing a tetrameric IFT88/70/52/46 complex obtained from the GSH elution. Elution volumes analyzed by SDS-PAGE are indicated with a bar below the chromatogram. c, HiLoad Superdex200 chromatogram and Coomassie Blue-stained SDS-polyacrylamide gel of the peak fractions showing a trimeric IFT70/52/46 and dimeric IFT70/IFT52(1–381) complex obtained from the GSH flow-through. Elution volumes analyzed by SDS-PAGE are indicated with a bar below the chromatogram.