Figure 2. T6SS mediated actin cross-linking correlates with endocytic uptake into host cells.
A. Gentamicin protection assay of various host cell lines exposed to various V52 strains at an MOI of 10 for 1 hour, followed by gentamicin treatment and enumeration of intracellular bacteria. Values are of triplicate wells and are expressed as percentage of initial inoculum recovered. Error bars indicate +/− 1 standard deviation. B. Actin cross-linking in host cells detected by western blot after exposure to various V52 strains at an MOI of 10 for 2 hours.
