Fig. 2.
Ubiquitin and α-syn aggregates colocalize with neuronal markers. Sections of hippocampus in 12-mo-old Gba1D409V/D409V brains, either pretreated with proteinase K (PK) (B) or not (A and C) before immunostaining. Immunostaining for NFH (A) exposed the presence of normal (arrowheads) and dystrophic (arrows) hippocampal neurites. (A) Colabeling of NFH and ubiquitin showed that ubiquitin aggregates were located in neurites. (B) Pretreating sections with proteinase K showed MAP2 colocalized with α-syn in Gba1D409V/D409V hippocampus. (C) Immunostaining for GFAP and ubiquitin showed no colocalization of the astrocyte marker with the ubiquitin aggregates. DAPI nuclear staining is shown in blue. (Scale bar, 50 μm.)