Fig. 7. Immunolocalization of Mast after microtubule depolymerization. S2 cells were grown for 0, 8 or 16 h in the presence of colchicine. Cells were stained to reveal Mast (red), and α-tubulin (A–C) or γ-tubulin (D) (green). Isolated chromosomes (E) were stained to reveal Mast (red) and Polo (green). DNA is shown in blue. Control cells (A) show a well-organized bipolar spindle and Mast localization to the spindle poles and the centromeres. In cells incubated in colchicine for short (8 h, B) or longer periods (16 h, C and D), microtubules depolymerize and Mast remains associated with both centrosomes (arrows) and centromeres. Mast staining co-localizes with γ-tubulin at the centrosomes (D) and with Polo at the centromeres (E). Bar = 5 µm.