Fig. 4.
miR-485 reduces spontaneous synaptic activity in hippocampal neurons. (A) Representative traces of spontaneous mEPSCs recorded from cultured hippocampal neurons transfected with negative control for the miR-M (a), miR-M (b), negative control for miR-I (c), and miR-I (d) showing a reduction in spontaneous mEPSC frequency after transfection with the mimic (b) and an increase following transfection with the inhibitor (d). (B) Quantitative analysis showing the transfection significantly changed mEPSP frequency [χ2(3,313) = 191.984, P < 0.001]. (C) Synaptic vesicle release (FM 1-43 destaining kinetics) was not affected by miR-485, but the number of recycled vesicles (amount of destaining) was reduced by miR-485 (yellow) and increased by the inhibitor (red) (P < 0.05). Destaining curves for negative controls (n = 8 coverslips with at least 60 boutons each) and for cultures treated with the miR-M (n = 8) and miR-I (n = 11) are shown.