Figure 5. Sca2 localizes to actin-associated bacterial surfaces and is sufficient to promote actin polymerization in cell extracts.
(a) Sca2 (white or green) was immunostained with anti-Sca2 antibodies, and actin (white or purple) was stained with Alexa 488-phalloidin in R. parkeriinfected Drosophila melanogaster S2R+ cells. Imaging was by deconvolution microscopy. Scale bar, 5 µm. (b) Magnified image of the bacterium in the lower left corner of a. Scale bar, 1 µm. (c) Timelapse micrographs of a cluster of polystyrene beads coated with GST-Sca2 and added to Xenopus laevis egg extract supplemented with rhodamine-actin. The elapsed time from adding beads to the extract is indicated (min:s). Scale bar, 20 µm.