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. Author manuscript; available in PMC: 2011 Jul 14.
Published in final edited form as: Nat Cell Biol. 2010 Oct 24;12(11):1057–1063. doi: 10.1038/ncb2109

Figure 2. Sca2 nucleates actin filaments.

Figure 2

(a) Polymerization of pyrene-actin over time with GST alone (160 nM, black line) or increasing concentrations of GST-Sca2 (indicated on left; green lines). Left panel: magnified view of the first 100 s of the time course shown on the right. Actin polymerization was detected by the increase in pyrene fluorescence that occurs on polymerization (AU, arbitrary units). (b) Effect of Sca2 concentration on the initial polymerization rate of pyrene-actin. Initial polymerization rate was assessed by measuring the gradient of the lines in the left graph of a. (c) Polymerization of pyrene-actin over time with GST-Sca2 alone (40 nM, green) or Sca2 and GST-RickA (40 nM and 200 nM respectively, black). (d) Polymerization of pyrene-actin over time with increasing concentrations of GST-Sca2-1106 (blue). Left panel: magnified view of the first 100 s of the time course shown on the right. (e) Polymerization of pyrene-actin over time with increasing concentrations of GST-Sca2-670 (blue). Left panel: magnified view of the first 100 s of the time course shown on the right. (f) Polymerization of pyrene-actin over time with increasing concentrations of GST-Sca2-646-1106 (red).