Skip to main content
. Author manuscript; available in PMC: 2012 Jul 14.
Published in final edited form as: Neuron. 2011 Jul 14;71(1):61–75. doi: 10.1016/j.neuron.2011.05.029

Figure 2. In vitro generation of SVZ ependymal clusters.

Figure 2

(A) IHC staining of in vitro cultured pRGPs from P0 mice under proliferating (3 days after plating, upper row) and differentiating conditions (10 days after plating, bottom row). Note γ-Tubulin (γ-Tub) staining showing single basal body during proliferation (arrows, corresponding to monociliated cells), and multiple basal bodies per cell after differentiation (arrows, corresponding to multiciliated cells). (B) Scanning electron microscopic (SEM) images of differentiated pRGPs showing multiciliated ependymal cells (ECs, *) arranged in clusters around monociliated cells (arrows). Right panels show higher magnification view of multiciliated cell (arrowhead), and monociliated cells (arrows). (C) IHC staining of ependymal clusters. Monociliated cell in the center is GFAP+ (*). Multiciliated ependymal cells show clustered γ-Tub staining (E), and their cell borders are represented by dash lines (traced from Phalloidin co-staining). (D) Stills from real-time live imaging movie of Foxj1-GFP+ pRGPs showing proliferation and differentiation of SVZ progenitors. Note the increase in GFP+ cell clustering during proliferation/differentiation transition (arrowheads, also see Movie S2). Scale bar: (A) 10 μm; (B) 10 μm / close-up 2 μm; (C) 5 μm; (D) 50 μm. See also Figure S2.