FIGURE 2.
Cytolytic mechanisms used by TB10.44–11-specific CD8+ T cells in vivo. A, The absolute number of TB10.4 4–11 tetramer+CD8+ T cells per spleen was determined and plotted vs TB10.44–11-specific killing for individual Mtb-infected B6 (●) or Pfn−/− (○) mice. Line, Nonlinear regression analysis for B6 (R2 = 0.97) and Pfn−/− (R2 = 0.99). Each data set is best fit by two separate curves, p = 0.0003. B, The absolute number of CFP1032–39 tetramer+CD8+ T cells per spleen was determined and plotted vs CFP1032–39-specific killing for individual Mtb-infected B10.BR (●) and B10.BR.Pfn−/− (○) mice. Line, Nonlinear regression analysis for B10.BR (R2 = 0.59) and B10.BR.Pfn−/− (R2 = 0.76) is shown. Each data set is best fit by two separate curves, p = 0.0012. C, CMTMR (CFSEneg) WT splenocytes, TB10.44–11-pulsed CFSElow WT, CFSEmid TNFR−/−, and CFSEhigh lpr splenocytes were mixed ~1:1:1:1 and injected into mice. Target cells recovered from the spleens of uninfected WT mice (top), WT (middle), and Pfn−/− (bottom) Mtb-infected mice and were analyzed by flow cytometry. Representative histograms are shown. D, The percent TB10.44–11specific killing was calculated for five mice per group and normalized to the average specific killing of WT targets in infected WT mice to determine the percent maximal killing for each target population (as described in Materials and Methods). Bar, mean ± SEM. **, p < 0.01 and ***, p < 0.001 by one-way ANOVA vs WT targets within the same recipient group or as indicated by the bracket. Data are representative of three similar experiments.