Figure 5. Viral titers and proinflammatory cytokine levels are similar between wildtype and PAD4-deficient mice.
Experimental setup as decribed in Figure 2. Lung homogenates from five mice per group were analyzed at d3 or d7 post infection with influenza A/WSN. (A) Viral titers were assessed by a plaque-forming assay on MDCK cells. Symbols represent individual animals (filled squares = PAD4 WT; open squares = PAD4 KO). Means +/− SEM are indicated. (B) Interleukin-6 levels were analyzed by tissue homogenate ELISA. Data is adjusted to starting tissue weight. Bars represent a group of eight mice as mean + SEM (white bars = PAD4 WT; grey bars = PAD4 KO). Data are representative of two individual experiments.