Fig. 1.
IL-1β cleavage and release from BM-DC. BM-DC were primed overnight with serial dilutions of LPS (10 ng/mL, 1 ng/mL, 0.1 ng/mL, 0 ng/mL). MF59 or alum were added to the cells at the indicated concentrations and cells were incubated for further 24 h. Cleavage of pro–IL-1β was assessed by Western blotting (A) (M: protein weight marker) and cytokine release into the culture supernatant was measured by multiplex-bead ELISA (B). BM-DC from Nlrp3−/− or WT mice were primed with 10 ng/mL of LPS and incubated overnight with MF59 (1:100, vol:vol) or alum (400 μg/mL) (C).