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. Author manuscript; available in PMC: 2011 Jul 7.
Published in final edited form as: Dev Cell. 2010 Sep 14;19(3):377–388. doi: 10.1016/j.devcel.2010.08.011

Figure 5. Rho-kinase is planar polarized in intercalating cells.

Figure 5

(A,B) Localization of Rho-kinase (HA:Rok, red) and F-actin (phalloidin, green) in stage 7 wild-type (A) or LatB-injected (B) embryos.

(C–L) Localization of Rho-kinase (Venus:RokK116A, red) and Baz (green). Stage 7 wild-type embryo (C) or embryos injected with baz dsRNA (E), LatB (G), eve and runt dsRNA (I), or Kr dsRNA (K). Quantitation of planar polarity in wild-type (D) and injected embryos (F,H,J,L). Rho-kinase planar polarity was not affected by baz RNAi (F) (n=6 wild-type Venus:RokK116A expressing embryos, 7 baz dsRNA-injected embryos, P=0.29). Rho-kinase planar polarity was reduced by injection of LatB (H) (5 embryos, P<0.001), eve and runt dsRNA (J) (7 embryos, P<0.001), or Kr dsRNA (L) (4 embryos, P=0.01). Scale bar = 10 μm.