Figure 2.
Induction of chemokine receptor (CCR)9 by mesenteric lymph node (MLN) dendritic cells (DCs) in vitro requires metabolism of exogenously derived retinol. (a, b) DCs were isolated from the MLN, pulsed with the ovalbumin (OVA) peptide (40 n), and cultured together with CFSE-labeled OT-I cells in RPMI or X-VIVO10 media with or without addition of fetal calf serum (FCS), UV-irradiated FCS, or retinal as indicated. CC chemokine receptor (CCR)9 expression on responding OT-I cells was assessed 3 days later. (a) Results are representative FACS plots from one experiment of four performed. (b) Results are representative FACS plots from one experiment of four (UV irradiated) and three (retinal) performed. CFSE, carboxyfluorescein; UV, ultraviolet.