Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Cancer Res. 2011 May 9;71(13):4707–4719. doi: 10.1158/0008-5472.CAN-10-4554

Figure 1. TGFϐ with TNFα generates tumor cells with a stable EMT phenotype that form heterogenous tumors in vivo.

Figure 1

A) Photomicrograph (upper) and flow cytometry analysis (bottom) of epithelial MMC and mesenchymal MMCTT and ETTM cells. B) RT-PCR of mRNA isolated from MMC, MMCTT, MMCTTE, ETTM and ANV5 cells using primers specific for E-cadherin, N-Cadherin, Zeb1, Twist, Snail and GAPDH. ANV5 is a stable BCSC cell line derived in our prior studies (11). C) Immunoblot of cells in (B) showing expression of E-Cadherin, N-Cadherin and β-actin. D) Flow cytometry analysis of N-Cadherin and E-Cadherin on ETTM cells, ETTM primarytumor and cultured cells. E) Expression of EMT-associated genes by qPCR analysis.