. 2010 Jun 30;20(2):269–287. doi: 10.1089/scd.2009.0501

Table 3.

Primers Used for Polymerase Chain Reaction

Gene	GenBank access No.	Forward primer, base pairs	Reverse primer, base pairs	cDNA, bp
RT-PCR
Nanog	NM_024865.2	ttccttcctccatggatctg 377–396	tctgctggaggctgaggtat 589–570	213
Oct4	NM_203289.3	agtgagaggcaacctggaga 518–537	gccggttacagaaccacact 642–623	125
Q-RT-PCR
Col type I alpha 1	NM_000088.3	tggcaaagaaggcggcaaagg 2829–2849	agcaccagcaggaccatcagc 2961–2941	133
Runx2	NM_004348.3	cccaggcagttcccaagcatttc 1475–1497	ggtagtgagtggtggcggacatac 1613–1590	139
ALP	NM_000478.3	gcaccgccaccgcctacc 592–609	ccacagatttcccagcgtccttg 741–719	150
BSP	NM_004967.3	agaggaggaggaagaagaggagac 326–349	gcccagtgttgtagcagaaagtg 437–415	112
Col type 2 alpha 1	NM_033150.2	gccccagaggtgacaaaggagag 3312–3334	aaggaccagcaggaccagaagc 3452–3431	141
Aggrecan	NM_001135.2	ggcatttcagcggttccttctcc 2400–2422	cagcagttgtctcctcttctacgg 2535–2512	136
Col type X alpha 1	NM_000493.3	ggacaacagggacccacaggag 571–592	tggacgaccaggagcaccatatc 693–671	123
Keratin 14	NM_000526.4	agatgattggcagcgtggaggag 1185–1207	ccagcaggcggcggtagg 1319–1302	135
Keratin 17	NM_000422.2	ttggcagcgtggaggagcag 1158–1177	ccagcaggcggcggtagg 1286–1269	129
Neurofilament	NM_021076.3	gcccaattcctttctcgcttccag 1318–1341	ggtctcctctgtctgttcctccac 1463–1440	146
α Feto-protein	NM_001134.1	gacccgaactttccaagccataac 740–763	gcagacaatccagcacatctcctc 882–859	143
α-1 antitrypsin	NM_001002236.2	ggagaggaagcacatcacccattg 2729–2752	agcaacaggcacaaagaagtcagg 2830–2807	102
GAPDH	NM_002046.3	gcctcccgcttcgctctctg 15–34	cgttgactccgaccttcaccttcc 130–107	116

Q-RT-PCR, quantitative-real-time-polymerase chain reaction.