Fig. 3.
Effects of BHA on ErPC3-induced cardiolipin oxidation, indicative of mitochondrial ROS generation, as determined with 10-N-Nonyl-Acridine Orange (NAO) by FACS. U118MG glioblastoma cells were treated with ErPC3 (7.5 μM) and/or BHA (200 μM) for 24 h. Control was treated with vehicle. The level of measured cardiolipin oxidation in the control is referred to as 1. The levels of cardiolipin oxidation in the experimental groups (BHA; ErPC3; (ErPC3) + BHA) are represented as relative to the control. These averages are derived from each time 4 independent experiments. ErPC3 enhanced cardiolipin oxidation. Addition of BHA to ErPC3 treatment reduced NAO labelling to control levels. BHA by itself reduced NAO labelling to below control levels. * P < 0.05 versus control; # P < 0.05 versus ErPC3