Figure 6.
Subcellular localization of the KJK/AtCSLD3 protein using GFP. Tobacco epidermis was infiltrated with Agrobacterium tumefaciens containing a binary vector with the 35S promoter and GFP fusion. (A–D) Confocal sections of transformed epidermal cells. KJK/AtCSLD3-GFP fusion protein is located in the endoplasmic reticulum (ER) (A). Successive 1-μm confocal sections (B,C,D) show that GFP fluorescence is located in the endoplasmic reticulum (arrows). A similar subcellular distribution was observed with the control ER-targeted GFP (E). The cytoplasmic GFP is distributed throughout the cytoplasm (arrow) restricted between the vacuole and the cell wall (F). (ER) Endoplasmic reticulum; (n) nucleus.