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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Cancer. 2007 Mar 1;109(5):957–965. doi: 10.1002/cncr.22479

FIGURE 3.

FIGURE 3

(A) Effect of treatment with PXR siRNA on the expression of P450 3A4 in OS187 cells. Cells were transfected with siRNA for PXR, along with siRNA scramble sequence or the sequence for fluorescent oligos “block-it” used as controls and to determine transfection efficacy, respectively. Total cell extracts were prepared for Western blotting and probed with anti-PXR antibody or anti-P450 3A4 antibody. The membranes were reprobed for actin. (B) Effect of treatment with PXR siRNA on the expression of P450 3A4 in HepG2 cells. Cells were transfected with siRNA for PXR. Total cell extract was prepared for Western blotting and probed with anti-PXR antibody or anti-P450 3A4 antibody. Membranes were reprobed for actin.