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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Dev Cell. 2011 Jun 14;20(6):775–787. doi: 10.1016/j.devcel.2011.04.018

Figure 2. Defective ventral neural patterning in E10.5Cdo−/−; Boc−/− and Gas1−/−; Boc−/− mouse embryos.

Figure 2

Immunofluorescent analysis of E10.5 forelimb level sections detects expression of Shh (green; A–H), FoxA2 (red; I–P), Nxk2.2 and Olig2 (red and green, respectively; Q–X) in wt (A, I, Q), Boc−/− (B, J, R), Cdo−/− (C, K, S), Cdo+/−; Boc−/− (D, L, T), Cdo−/−; Boc−/− (E, M, U), Gli2−/− (F, N, V), Gas1−/− (G, O, W), and Gas1−/−; Boc−/− (H, P, X) embryos. Arrowheads denote FoxA2 and Nkx2.2 double positive floorplate cells in Cdo−/− embryos (S), but not Boc−/− embryos (R). Despite the complete loss of FoxA2+ FP cells in both Cdo−/−; Boc−/−, and Gli2−/− embryos (M and N, respectively), there is a selective loss of Olig2+ but not Nkx2.2+ cells in Cdo−/−; Boc−/−embryos (U). In contrast, Gli2−/− embryos lack Nkx2.2+ cells, but maintain Olig2 expression (V). Gas1−/−; Boc−/− embryos display complete loss of FoxA2 (P), Nkx2.2 and Olig2 (X) at E10.5. Scale bar: A, 50µm. See Figure S1 for a more detailed analysis of neural patterning in Boc−/−embryos. Refer to Figure S2 for Gas1, Cdo and Boc protein distribution in E10.5 mouse neural tubes.