Figure 2.
CD4+ CD25high FoxP3+ Treg cells (iTreg) are induced from naive CD4+CD45RA+ T helper (Th0) cells primed with N-CMFs. Th0 cells were cultured without or with allogeneic N-CMFs at a ratio 1:10 for one week. (A) The RNA from the N-CMF primed Th0 cells was analyzed for FoxP3 mRNA expression using real time RT-PCR. The mRNA levels for FoxP3 was normalized to 18S. Data represent mean of mRNA Δ fold increase ± SEfrom duplicates in four experiments (n=8) *P<0.01. (B) The surface CD25 and intracellular FoxP3 expression in the allogeneic N-CMF or BM-derived DC primed CD4+ T cells was analyzed using flow cytometry.. The appropriate isotype controls were included in the experiments. A representative experiment is shown (n=5). *p<0.01.