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. Author manuscript; available in PMC: 2011 Jun 3.
Published in final edited form as: Nat Biotechnol. 2010 Dec 12;29(1):68–72. doi: 10.1038/nbt.1732

Figure 1.

Figure 1

Selective labeling of 5-hmC in genomic DNA. (a) The hydroxyl group of 5-hmC in duplex DNA can be glucosylated by β-GT to form β-glucosyl-5-hydroxymethylcytosine (5-gmC) using UDP-Glu as a cofactor. (b) An azide group can be installed onto 5-hmC using chemically modified UDP-Glu (UDP-6-N3-Glu), which in turn can be labeled with a biotin moiety using click chemistry for subsequent detection, affinity purification and sequencing.