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Structure-guided biochemical analyses of p300:MEF2 interactions. (A) Diagram of the biochemical assay. (B) Luciferase reporter assay of p300 TAZ2 mutants. Columns 1–3 are controls. Column 4 is from wild-type p300 and MEF2 interaction. Columns 5–8 are IF-I mutants, 10–14 are IF-II mutants and 16–19 are IF-III mutants. Column 6 is a combined mutant of IF-I and IF-II.
