Fig. 2.
Phylogenetic relations of ccr genes. The ccrA and ccrB genes from the following strains were used. DDBJ/EMBL/GenBank database accession numbers are indicated in parentheses, and species are not indicated in the cases of S. aureus strains. ccrA1 and ccrB1, NCTC10442 (AB033763), MSSA476 (BX571857), and GIFU12263 (AB063171); ccrA2 and ccrB2, N315 (D86934), CA05 (AB063172), and MW2 (NC003923); ccrA3 and ccrB3, 85/2082 (AB037671); ccrA4 and ccrB4, HDE288 (AF411935), ATCC 12228 (AE015929), CHE482-1 (EF126185), and CHE482-2 (EF126186); ccrA1 and ccrB6, ATCC 15305 (NC007350); ccrA1 and ccrB7, TSU33 (AB353724); ccrA5 and ccrB3, KM241 (AM904731); and ccrC1, WIS (AB121219), TSGH17 (AY894416), 85/2082 (AB037671), M (U10927), S. haemolyticus JCSC1435 (AP006716), S. haemolyticus 25-60 (EF190467), S. epidermidis 13-48 (EF190468), P1 (AB656125), and S. saprophyticus ATCC 15305. The nucleotide sequences of 14 ccrA genes, 14 ccrB genes, and 9 ccrC genes were aligned by using the ClustalX program. The phylogenetic tree was generated by the neighbor-joining method by creating 2,000 bootstrap replicates. The tree was visualized with TreeView software, which was obtained from the website http://taxonomy.zoology.gla.ac.uk/ROD/treeview.html. The branch length indicates the distance, which is expressed as the number of substitutions per 100 bases.