Figure 5.
SMER28 induced cocompartmentalization of APP-CTF and LC3-II. A) N2a-APP cells were treated for 6 h with SMER28 (50 μM), and whole-cell lysates were fractionated by sucrose gradient. An aliquot of each of the 12 fractions recovered was analyzed by SDS-PAGE and Western blotting for APP-CTF, LC3, γ-adaptin, and Bip. B) N2a-APP cells were transfected with an LC3-EGFP-containing plasmid; at 16 h post-transfection, cells were treated for 6 h with SMER28 (50 μM). Cells were fixed and immunostained with RU-369, an APP C-terminal antibody, or APLP1 antibody and imaged by confocal microscopy.