Figure 3.
NF-κB p65 expression in astrocytes and microglia. (A) Percentage of GFAP stained astrocytes with anti-p65 nuclear staining. (B) Percentage of CD11b stained microglia with anti-p65 nuclear staining. (C) Percentage of CD11c stained (highly activated) microglia with anti-p65 nuclear staining. (D) A representative microglial cell with nuclear NF-κB p65 staining with anti-CD11c (green), anti-p65 (red), and DAPI (blue). Scale bar = 20 μm. (E) The number of CD11c positive microglia found in 20 fields of random sampling. Blue bars represent samples from the UK cohort, red bars samples from the US cohort. Sample IDs prefixed “A” are from ASC donors, prefixed “C” from control donors.