Figure 4.

Immunohistochemistry images of tumors excised from vehicle- and RAMH-treated mice (× 40). A: Proliferating cholangiocytes (PCNA) and apoptotic cholangiocytes (Caspase 3). RAMH induced a decrease in PCNA-positive cholangiocytes coupled with an increase in apoptotic cholangiocytes compared to vehicle-treated mice; B: VEGF-A and VEGF-R2. RAMH treatment caused a decrease in the expression of both VEGF-A and VEGF-R2.