Figure 8.
After demyelination, CCR2 signaling was not activated in the spinal cord. A, MCP1∷MCP1–mRFP1; CCR2∷CCR2–EGFP mice were subjected to LPC-induced demyelination of the sciatic nerve. There was no expression of MCP1 or CCR2 at a detectable level. Leukocytes outside the spinal cord were clearly visible (green arrow). B–D, CCR2 expression was also examined at the mRNA level by in situ hybridization. The spinal cord does not contain significant CCR2-expressing cellular components (B), whereas many cells in the sciatic nerve (C) and DRG (D) express CCR2 receptors in the LPC group. DH, Dorsal horn; DC, dorsal column. Scale bars, 60 μm.