Fig. 2. Drug resistance analysis of Abcc10−/− mouse embryo fibroblasts.
A) Analysis of Abcc10 protein expressed in MEFs. Membranes (20 µg) prepared from wild-type (WT103.2) and Abcc10 null mice (KO95.2) were separated by SDS-PAGE and analyzed with Abcc10 antibody. The positive control (left) is ABCC10-transfected HEK293 cells (20 µg). B), C) Drug sensitivity of Abcc10 null and wild-type MEFs to various agents. MEFs generated from C57BL/6x129 (B) and C57BL/6 (C) wild-type and Abcc10 null mice were analyzed for sensitivity to the indicated agents using an MTS/PMS assay. In B, the cell lines are WT103.2 (wild-type) and KO95.2 (knock out); in C, WT3-3 (wild-type) and BKO7 (knockout). D) Accumulation of paclitaxel in wild-type (WT103.2) and Abcc10−/− (KO95.2) MEFs. Cells (C57BL/6x129 - WT103.2, KO95.2; C57BL/6 - WT3-3, BK07 were incubated in the presence of 0.1µM [3H]paclitaxel and accumulation was measured at 30 minutes. Data are means +/− SDs. B – C, * P < 0.05 as assessed by the Two–tailed Wilcoxon test. *;D, P < 0.05 as assessed by the Wilcoxon two sided, two sample test.