Fig. 4.
(A) MiR-146b-5p expression in U87-MG xenographs in nude mouse is lower in invasive cells compared to primary tumor mass. Laser capture of tumor tissue demonstrating different regions of capture: 1) tumor core, 2) tumor boundary, 3) invasive tumor. (Subsequently stained with H&E for visualization.) (B) Real-time RT-PCR analysis of miR-146b-5p expression in U87-MG cells excised by LCMD from 4 tumor-bearing mice (p**<0.01 vs. tumor core). (C, D) MTT cell viability assay indicated miR-146b-5p did not significantly decrease cell viability in U87-MG (C) or U251 (D) cells 48, 72, or 96 hours post-transfection. (E, F) Cell growth assay revealed miR-146b-5p transfection resulted in reduced U87-MG (E) and U251 (F) cell numbers after 96 hours in culture (*p<0.05 vs. non-transfected control, #p<0.05 v.s scrambled miRNA control).