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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Eur J Pharmacol. 2011 Feb 16;659(2-3):139–145. doi: 10.1016/j.ejphar.2011.01.066

Figure 4. Prolonged treatment with CP47,497-C8 or JWH073 promotes CB1 receptor internalization.

Figure 4

(A) In CB1 expressing HEK293 cells 100nM CP47,497-C8, JWH073 or JWH018 treatment results in robust receptor internalization (3 replicate samples from 5-14 independent experiments, except for 360 min time point: 3–4 experiments). (B) Following 2 hours of exposure to increasing concentrations of each drug, CB1 internalized in a concentration-dependent manner (3 replicate samples from 4 to 14 independent experiments). (C) 1 μM rimonabant prevents receptor internalization by each drug treatment. Values are presented as mean ± S.E.M. Data in (A) and (B) analyzed using one-way ANOVA with Bonferroni's multiple comparison test. Data in (C) analyzed using unpaired Student's t-test. *P < 0.05, ** P < 0.01, *** P < 0.0001.