Fig. 6.
Effects of IFN-γ and IL-4 deletion on the expression of selected caMac and aaMac genes by Mac from uninfected and C. neoformans-infected mice. RNA from enriched Mac isolates was analyzed at 3 wpi. (A) RT PCR mRNA analysis of RNA from iNOS, Arg1, and Fizz1 was performed as described in the legend for Fig. 1. The band photographs are representative of 3 independent experiments. (B) RNA from each sample was subjected to semiquantitative RT PCR analysis for Arg1 and Fizz1, and expression was normalized to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) housekeeping gene expression within each sample. The average relative expression of each gene in IL-4−/− cells in each independent experiment was used to calculate the relative expression ratios for WT and IFN-γ−/− cells. The graph is representative of 3 or more experiments, where each sample was run in triplicate (n = 3 mice/group).