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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Immunity. 2011 Apr 22;34(4):541–553. doi: 10.1016/j.immuni.2011.04.006

FIGURE 3. HP-IL-7 induced mTOR activity is essential for T-bet mediated functional maturation.

FIGURE 3

(A – F) Naïve WT or Tbx21−/− OT-1 cells were detected by flow cytometry on day 5 after transfer into intact or irradiated (700 rads) recipients that were either untreated (A,D,E,F) or treated with anti-IL-7Rα (100 µg) on day -1 and day 2 (B), or with rapamycin (0.75 mg/Kg/day) (C) and evaluated for; (A) CFSE dilution, T-bet and CD122 expression. (B and C) T-bet and CD122 expression in recipients treated with anti-IL-7Rα (B) or in radiated recipients treated with rapamycin (C). (D) The expression of CD122 in WT or Tbx21−/− OT-1 cells. (E) CFSE dilution of WT or Tbx21−/− OT-1 cells. (F) The number of OT-1 (CD8α+Thy1.1+) cells (left), percentage of IFN-γ+ OT-1 cells (middle), and in vivo cytolysis (right). The error bars are SD of three independently obtained values. The data shown is representative of three experiments (* p<0.05; **p<0.01; n.s., not significant).