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. 2011 Apr 18;121(5):2025–2036. doi: 10.1172/JCI43802

Figure 4. Analysis of blood monocyte numbers and phenotype following apoE complementation.

Figure 4

(A) Total monocytes and monocyte subsets were quantified by flow cytometry in blood of baseline mice or 4 weeks after infection with ad-Empty and ad-hApoE3 (n = 10–34 mice per bar). (B) CD11b and (C) CD62L (Ly-6Chi monocytes only) expression was analyzed with (black bars) or without apoE complementation (white bars) of Apoe–/– mice maintained on a HFD. n = 5–9 mice per bar; *P < 0.01. (D) Flow plot overlays show whole leukocyte fraction in blood (gray) overlaid with profiles of Ly-6Clo monocytes (black) from Apoe–/– mice fed a HFD followed by apoE complementation or no complementation. (E) Summary of relative changes in SSC-A shifts in Ly-6Clo monocytes from Apoe–/– mice on a HFD complemented (black bar) or not (white bars) with apoE-encoding vector. Data are compared with SSC-A of monocytes from Apoe+/+ control mice. (F) Effect of hApoE3 complementation on surface expression of CD115. n = 5 mice per bar; **P < 0.001.