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. 2011 Apr 18;121(5):2025–2036. doi: 10.1172/JCI43802

Figure 2. CCR7-independent removal of Apoe–/– plaque macrophages after apoE complementation.

Figure 2

Apoe–/–Ccr7–/– and Apoe–/–Ccr7+/+ (littermate control) mice were fed HFD for 9 weeks. A cohort of animals from each genotype was sacrificed to establish baseline (white and gray bars). A second cohort of Apoe–/–Ccr7–/– was then infected with ad-hApoE3 vector for 4 weeks (black bar) before sacrifice. (A) Plaque area was quantified in aortic sinus sections and (B) en face aortic arch preparation. (C) Lipid area was measured by ORO coloration and (D) macrophage content by CD68+staining. (E) Representative photomicrographs of macrophage (CD68+) area in sections of the aortic sinus at time of sacrifice in each group. Green, CD68; blue, DAPI. Original magnification, ×50. Data represent mean ± SEM from 2 independent experiments; n = 3 mice in Apoe–/–Ccr7+/+ baseline group, n = 5 in Apoe–/–Ccr7–/– baseline group, n = 6 in Apoe–/–Ccr7–/– ad-hApoE3 group; ***P < 0.001.