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. 2011 Apr 15;22(8):1312–1320. doi: 10.1091/mbc.E10-08-0723

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© 2011 Teng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Gfer expression modulates total and nuclear p27^kip1 in KLS cells. Digital optical section (Apotome; 630×) immunocytochemistry images depicting p27^kip1 (i–iii), Jab1 (iv–vi), and Gfer (vii–ix) levels in LacZ and Gfer KD and MSCV-Gfer KLS cells. Protein levels were detected by probing with secondary antibodies conjugated to Cy3 (red) fluorophore. DAPI is shown in blue, and average signal intensities ± SD calculated from at least 250 cells from five independent experiments are indicated in green. *p < 0.05.