FIGURE 2:
KD of Gfer in KLS cells results in enhanced proliferative capacity. (A) Apoptosis assessed in resorted GFP+ LacZ and Gfer-KD KLS cells by measuring Annexin V and 7AAD reactivity. Representative histograms with average percentage of early and late apoptotic cells ± SD from n = 3 experiments are shown; p = 0.3. (B) TEM images, 25,000× magnifications, depicting the ultrastructural details in LacZ and Gfer-KD KLS cells. Scale bars represent 0.5 μm. (C) Average (n = 3) cumulative cell yield from indicated genotypes during in vitro proliferation assays performed in Terasaki plates for 8 d in serum-free medium; *p < 0.05. (D) Representative histograms (n = 2) indicating intracellular ROS levels in LacZ and Gfer KD KLS cells on days 6 and 8 of the proliferation assay. Measurements were based on the production of oxidized R6G by the intracellular oxidation of the nonfluorescent dihydro-R6G. The percentage of cells (y-axis) and oxidized R6G fluorescence intensities (x-axis) in Lacz and Gfer KD KLS cells are shown.