Figure 2. Protein encapsulation and release from ICMVs.
a, Encapsulation efficiency of the globular proteins SIV-gag, FLT-3L, or OVA in lipid vesicles collected at each step of ICMV synthesis. b, c, Comparison of OVA encapsulation efficiency (b), and total protein loading per particle mass (c) in ICMVs vs. dehydration-rehydration vesicles (DRVs) or PLGA nanoparticles. d, Kinetics of OVA release from simple liposomes, MLVs, or ICMVs (all with base lipid composition 4:5:1 DOPC:MPB:DOPG) incubated in RPMI medium with 10% serum at 37°C measured over 30 days in vitro. Also shown for comparison are release kinetics for liposomes stabilized with cholesterol and PEG-lipid (38:57:5 DOPC:chol:PEG-DOPE). e, Release of OVA from ICMVs was measured in buffers simulating different aspects of the endolysomal environment: reducing buffer, 100 mM β-mercaptoethanol (β-ME) in PBS; acidic buffer, 50 mM sodium citrate pH 5.0; lipase-containing buffer, 500 ng/mL lipase A in PBS. Data represent the mean ± s.e.m of at least three experiments with n = 3.