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M2 macrophage polarization by flAcrp is dependent on AMPK in Kupffer cells from ethanol- and pair-fed rats. Kupffer cells isolated from ethanol- and pair-fed rats were transfected or not with 100 nm of AMPK siRNA or scrambled siRNA and then cultured with or without 45 nm flAcrp for 18 h. Arg-1 and 18 S mRNA measured by qRT-PCR. n = 4, values with different superscripts (a–d) within each treatment group are significantly different from each other (p < 0.05).
