FIGURE 4.
Differential effects of gAcrp and flAcrp on expression of M2 macrophage markers in RAW264.7 macrophages. A–I, RAW264.7 macrophages were treated either without or with 4.5–45 nm gAcrp or 4.5–45 nm flAcrp for 18 h. A–H, expression of markers of M2 polarization measured by qRT-PCR. mRNA of interest was normalized to 18 S mRNA. n = 4. + indicates p < 0.05 compared with cells not treated with adiponectin; * indicates p < 0.05 flAcrp-treated cells compared with gAcrp-treated cells. I, surface expression of CD206 was measured by flow cytometry. Histogram is representative of four independent experiments.